Journal: Anti-Cancer Drugs
Article Title: Oxaliplatin facilitates tumor-infiltration of T cells and natural-killer cells for enhanced tumor immunotherapy in lung cancer model
doi: 10.1097/CAD.0000000000001248
Figure Lengend Snippet: Oxaliplatin treatment induces the concentrations of chemokines in tumor tissues. Murine Lewis lung carcinoma (LLC) was established and treated as indicated above. Concentrations of chemokines including CXCL9 (a), CXCL10 (b), and CXCL11 (c) in the treated tumor tissues were examined using ELISA. The expressions of CXCR3 (the receptor of CXCL9/10/11) on myeloid cell (d), T cells (e), and NK cells (f) were examined using flow cytometry. Data represent means ± SD. n = 6 mice. ** P < 0.01, *** P < 0.001.
Article Snippet: After blocking with 5% nonfat milk, the membranes were incubated with the primary antibodies specific for CXCL9, CXCL10 and CXCL11 (Santa Cruz, 1:1000 dilution, 4°C overnight).
Techniques: Enzyme-linked Immunosorbent Assay, Flow Cytometry